Isothermal High Temperature Range

IsoPol^® BST⁺ shows enhanced strand displacement activity compared to Bst polymerase. Activities at different concentrations of KCl at 37°C relative to activity at 50 mM KCl are shown for each polymerase.

IsoPol® BST⁺ tolerates higher concentrations of salt compared to Bst polymerase. Polymerase activity of Bst and IsoPol™ BST⁺ as function of NaCl data represented relative to highest activity of each polymerase.

IsoPol BST⁺ has superior strand displacement activity compared to Bst LF. SDA was measured at 37°C using a nicked dsDNA template.

IsoPol BST⁺ has enhanced processivity compared to Bst LF. Processivity was determined at 37°C using a primed circular ssDNA.

IsoPol BST⁺ results in faster detection of target in both LAMP and RT-LAMP. When IsoPol BST⁺ was used in LAMP and RT-LAMP, time to results were strongly reduced compared to the non-engineered backbone, Bst LF. IsoPol BST⁺also significantly outperformed engineered versions of Bst LF from another vendor (Bst Vendor A). LAMP was used to detect 0.05 ng of λ DNA (Fig 5).RT-LAMP was performed with AMV RT to detect 10 ng of MS2 RNA template (Fig 6). LAMP and RT-LAMP were performed at 65°C.

IsoPol BST⁺ polymerase has high tolerance to matrix effects. LAMP was performed using λ DNA template in presence of human serum demonstrating that IsoPol BST⁺ maintains high activity over a wide range of serum concentrations. No amplification was detected in the absence of template.